Efficacy of a Next Generation Quaternary Ammonium Chloride Sanitizer on Staphylococcus and Pseudomonas Biofilms and Practical Application in a Food Processing Environment

Foodborne pathogens are known to adhere strongly surfaces and can form biofilms in food processing facilities; therefore, their potential contaminate manufactured foods underscores the importance of sanitation. The objectives this study were (1) examine efficacy a new-generation sanitizer (Decon7) on Staphylococcus Pseudomonas biofilms, (2) identify biofilm bacteria from workers’ boots relation previous chemistry, (3) validate Decon7 an abattoir/food environment, (4) compare sensitivity isolated boot new- early (Bi-Quat)-generation QAC sanitizers. was applied at two concentrations (5%, 10%) shown be effective within 1 min exposure against enhanced spp. 96-well microplates. also used treat that had accumulated high levels due ineffective sanitization. Bacteria before enzyme/sanitizer treatment identified through 16S rRNA PCR DNA sequencing. All treatments carried out triplicate analyzed by one-way RM-ANOVA or ANOVA using Holm–Sidak test for pairwise multiple comparisons determine significant differences (p < 0.05). data show difference between untreated control groups. There ~4–5 log reduction (microplate assay) first > 3-log bacterial population observed boots. new next-generation sanitizers more than prior sanitizers, enzyme pre-treatment facilitate penetration contact surfaces. rotation chemistries may prevent selective retention chemistry-tolerant microorganisms facilities.